Your Favourite Protein (YFP)

Question 1. Your Favourite Protein (YFP) has just been identified to interact with a novel, previously uncharacterised protein by immunoprecipitation and mass spectrometry. Discuss and evaluate methods or techniques support this interaction. (4 or 5 methods are enough) examples: 1- Tandem Affinity Purification- TAP tagging 2-Yeast Two Hybridization(Y2H) 3-Protein Microarray 4-BiFC (Biomolecular Fluorescence Complementation) (500 words or less, worth 10 marks) Question 2. Discuss the advantages and disadvantages of using Yeast-2-Hybrid to study proteinprotein interactions (300 words or less, worth 5 marks). Question 3. Discuss one method that you could use to study protein and nucleic acid interactions. Discuss the advantageous and disadvantageous of this method (300 words or less, worth 5 marks).